7u0y

X-ray diffraction
2.66Å resolution

Crystal structure of Pepper RNA aptamer in complex with HBC599 ligand and Fab BL3-6

Released:
Source organism: Mus musculus

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Fab BL3-6 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Fab BL3-6 light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
RNA aptamer Chain: R
Molecule details ›
Chain: R
Length: 67 nucleotides
Theoretical weight: 21.73 KDa

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 61.247Å b: 96.071Å c: 148.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.255
Expression system: Escherichia coli