X-ray diffraction
2.95Å resolution

Crystal structure of the human mitochondrial seryl-tRNA synthetase (mt SerRS) bound with a seryl-adenylate analogue


Function and Biology Details

Reaction catalysed:
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Serine--tRNA ligase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 54.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9NP81 (Residues: 35-518; Coverage: 93%)
Gene names: SARS2, SARSM
Sequence domains: tRNA synthetase class II core domain (G, H, P, S and T)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: R3
Unit cell:
a: 150.59Å b: 150.59Å c: 142.47Å
α: 90° β: 90° γ: 120°
R R work R free
0.24 0.237 0.285
Expression system: Escherichia coli BL21(DE3)