› 7tyi

Electron Microscopy
3.3Å resolution

Calcitonin Receptor in complex with Gs and rat amylin peptide, CT-like state

Released:
DOI: 10.2210/pdb7tyi/pdb
PDB entry 7tyi coloured by chain, front view.
PDB entry 7tyi coloured by chain, side view.
PDB entry 7tyi coloured by chain, top view.
Source organisms:
Primary publication:
A structural basis for amylin receptor phenotype.
Cao J, Belousoff MJ, Liang YL, Johnson RM, Josephs TM, Fletcher MM, Christopoulos A, Hay DL, Danev R, Wootten D, Sexton PM
Science 375 eabm9609 (2022)
PMID: 35324283
Related structures: EMD-26180

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210535 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.69 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 138 amino acids
Theoretical weight: 15.14 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Islet amyloid polypeptide Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 3.92 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P12969 (Residues: 38-74; Coverage: 53%)
Gene name: Iapp
Sequence domains: Calcitonin / CGRP / IAPP family
Calcitonin receptor Chain: R
Molecule details ›
Chain: R
Length: 501 amino acids
Theoretical weight: 58.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P30988 (Residues: 25-474; Coverage: 100%)
Gene name: CALCR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PLM 10 x PLM
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-26180
Expression systems:
  • Trichoplusia ni
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Metabolic hormone action in the VTA: Reward-directed behavior and mechanistic insights.
Geisler et al. (2023)
4 more

2 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
1 more