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Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 6YF

X-ray diffraction
1.805Å resolution
PDB entry 7tl4 coloured by chain, front view.
PDB entry 7tl4 coloured by chain, side view.
PDB entry 7tl4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA primase large subunit
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Multimeric state: monomeric
Accessible surface area: 9378.73 Å2
Buried surface area: 793.13 Å2
Dissociation area: 215.82 Å2
Dissociation energy (ΔGdiss): 16.56 kcal/mol
Dissociation entropy (TΔSdiss): 1.42 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148983

Macromolecules

DNA primase large subunit
Chain: A
Length: 201 amino acids
Theoretical weight: 23.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20457 (Residues: 316-512; Coverage: 37%)
Gene names: PRI2, YKL045W, YKL258
Pfam: Eukaryotic and archaeal DNA primase, large subunit
InterPro: DNA primase large subunit, eukaryotic/archaeal
DNA primase large subunit in PDB entry 7tl4, assembly 1, front view.
DNA primase large subunit in PDB entry 7tl4, assembly 1, side view.
DNA primase large subunit in PDB entry 7tl4, assembly 1, top view.
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