› 7tj7

Electron Microscopy
8Å resolution

Cardiac thin filament decorated with C1 Ig-domain and regulatory M-domain of cardiac myosin binding protein C (cMyBP-C)

Released:
DOI: 10.2210/pdb7tj7/pdb
PDB entry 7tj7 coloured by chain, front view.
PDB entry 7tj7 coloured by chain, side view.
PDB entry 7tj7 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-electron microscopy reveals cardiac myosin binding protein-C M-domain interactions with the thin filament.
Risi CM, Villanueva E, Belknap B, Sadler RL, Harris SP, White HD, Galkin VE
J Mol Biol 167879 (2022)
PMID: 36370805
Related structures: EMD-25918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-221261 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 375 amino acids
Theoretical weight: 41.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P68137 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Myosin-binding protein C, cardiac-type Chains: G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O, P, Q, R
Length: 220 amino acids
Theoretical weight: 24.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14896 (Residues: 151-370; Coverage: 17%)
Gene name: MYBPC3
Sequence domains:
tropomyosin model Chains: S, T, U, V
Molecule details ›
Chains: S, T, U, V
Length: 135 amino acids
Theoretical weight: 11.51 KDa
Source organism: Sus scrofa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8Å
Relevant EMDB volumes: EMD-25918
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structure of the native myosin filament in the relaxed cardiac sarcomere.
Tamborrini et al. (2023)
1 more