7t93

X-ray diffraction
1.65Å resolution

Crystal Structure of Fumarate hydratase class II from Mycobacterium ulcerans in complex with L-Malate

Released:
Source organism: Mycobacterium ulcerans Agy99
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 496 amino acids
Theoretical weight: 52.47 KDa
Source organism: Mycobacterium ulcerans Agy99
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0PKR5 (Residues: 1-475; Coverage: 100%)
Gene names: MUL_0181, fum, fumC
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 98.04Å b: 120.82Å c: 158.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.138 0.161
Expression system: Escherichia coli BL21(DE3)