X-ray diffraction
2.25Å resolution

E. coli dihydroorotate dehydrogenase bound to the inhibitor HQNO


Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Dihydroorotate dehydrogenase (quinone) Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 40.29 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli B
  • Canonical: P0A7E1 (Residues: 1-336; Coverage: 100%)
Gene names: JW0928, b0945, pyrD
Sequence domains: Dihydroorotate dehydrogenase

Ligands and Environments

Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 104.046Å b: 169.328Å c: 129.97Å
α: 90° β: 90° γ: 90°
R R work R free
0.218 0.217 0.232
Expression system: Escherichia coli B