› 7sy9

X-ray diffraction
2.75Å resolution

Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1

Released:
DOI: 10.2210/pdb7sy9/pdb
PDB entry 7sy9 coloured by chain, front view.
PDB entry 7sy9 coloured by chain, side view.
PDB entry 7sy9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoylalanine--D-glutamate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 48.31 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HVZ9 (Residues: 1-1, 8-448; Coverage: 99%)
Gene names: PA4414, murD
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P6522
Unit cell:
a: 94.75Å b: 94.75Å c: 442.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.247
Expression system: Escherichia coli BL21(DE3)

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