› 7sx9

Electron Microscopy
10Å resolution

T-Plastin-F-actin complex, anti-parallel bundled state

Released:
DOI: 10.2210/pdb7sx9/pdb
PDB entry 7sx9 coloured by chain, front view.
PDB entry 7sx9 coloured by chain, side view.
PDB entry 7sx9 coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism for bidirectional actin cross-linking by T-plastin.
Mei L, Reynolds MJ, Garbett D, Gong R, Meyer T, Alushin GM
Proc Natl Acad Sci U S A 119 e2205370119 (2022)
PMID: 36067297
Related structures: EMD-25495

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146768 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, E, G, H
Molecule details ›
Chains: A, B, C, E, G, H
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P68139 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Plastin-3 Chain: D
Molecule details ›
Chain: D
Length: 630 amino acids
Theoretical weight: 70.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13797 (Residues: 1-630; Coverage: 100%)
Gene name: PLS3
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 6 x MG
1 modified residue:
Ligand HIC 6 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 10Å
Relevant EMDB volumes: EMD-25495
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Actin Bundles Dynamics and Architecture.
Rajan et al. (2023)
1 more