› 7swf

Electron Microscopy
3.79Å resolution

Cryo-EM structure of Arabidopsis Ago10-guide-target RNA complex in a central duplex conformation

Released:
DOI: 10.2210/pdb7swf/pdb
PDB entry 7swf coloured by chain, front view.
PDB entry 7swf coloured by chain, side view.
PDB entry 7swf coloured by chain, top view.
Source organisms:
Primary publication:
The molecular mechanism of microRNA duplex selectivity of Arabidopsis ARGONAUTE10.
Xiao Y, MacRae IJ
Nucleic Acids Res (2022)
PMID: 35801914
Related structures: EMD-25472

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195020 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Protein argonaute 10 Chain: A
Molecule details ›
Chain: A
Length: 988 amino acids
Theoretical weight: 110.98 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9XGW1 (Residues: 1-988; Coverage: 100%)
Gene names: AGO10, At5g43810, MQD19.17, PNH, ZLL
Sequence domains:
RNA (5'-R(P*UP*GP*GP*AP*GP*UP*GP*UP*GP*AP*CP*AP*AP*UP*GP*GP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.79 KDa
Source organism: unidentified
Expression system: Not provided
RNA (5'-R(P*CP*CP*AP*UP*UP*GP*UP*CP*AP*CP*AP*CP*UP*CP*CP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.64 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.79Å
Relevant EMDB volumes: EMD-25472
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

1 review citation

Beyond Loading: Functions of Plant ARGONAUTE Proteins.
Liang et al. (2023)
2 other articles

2 mentions without citation

When Argonaute takes out the ribonuclease sword.
Nakanishi K. (2023)
1 more