› 7sw1

Electron crystallography
1.85Å resolution

MicroED structure of proteinase K from a 115 nm thick lamella measured at 200 kV

Released:
DOI: 10.2210/pdb7sw1/pdb
PDB entry 7sw1 coloured by chain, front view.
PDB entry 7sw1 coloured by chain, side view.
PDB entry 7sw1 coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Benchmarking the ideal sample thickness in cryo-EM.
Martynowycz MW, Clabbers MTB, Unge J, Hattne J, Gonen T
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34873060
Related structures: EMD-25459

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.85Å
Relevant EMDB volumes: EMD-25459

Quick links

Citations

5 review citations

Bringing Structure to Cell Biology with Cryo-Electron Tomography.
Young et al. (2023)
4 more