› 7sv2

X-ray diffraction
2.46Å resolution

Human Cytochrome P450 (CYP) 3A5 ternary complex with azamulin

Released:
DOI: 10.2210/pdb7sv2/pdb
PDB entry 7sv2 coloured by chain, front view.
PDB entry 7sv2 coloured by chain, side view.
PDB entry 7sv2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of the homotropic cooperative binding of azamulin to human cytochrome P450 3A5.
Hsu MH, Johnson EF
J Biol Chem 298 101909 (2022)
PMID: 35398097

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 3A5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 480 amino acids
Theoretical weight: 54.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20815 (Residues: 22-22, 24-497; Coverage: 95%)
Gene name: CYP3A5
Sequence domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand MWY 8 x MWY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: C2221
Unit cell:
a: 104.786Å b: 134.3Å c: 275.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.223
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Differential Effects of Clotrimazole on X-Ray Crystal Structures of Human Cytochromes P450 3A5 and 3A4.
Hsu et al. (2023)
1 more

1 mention without citation

Structural characterization of the homotropic cooperative binding of azamulin to human cytochrome P450 3A5.
Hsu et al. (2022)