X-ray diffraction
2.89Å resolution

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
p1a Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 35.88 KDa
Source organism: Bat SARS CoV Rf1/2004
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q0QDZ2 (Residues: 1536-1850; Coverage: 7%)
Sequence domains: Coronavirus papain-like peptidase

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6422
Unit cell:
a: 176.306Å b: 176.306Å c: 79.834Å
α: 90° β: 90° γ: 120°
R R work R free
0.203 0.201 0.24
Expression system: Escherichia coli BL21(DE3)