› 7skr

X-ray diffraction
2.89Å resolution

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p1a Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 35.88 KDa
Source organism: Bat SARS CoV Rf1/2004
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0QDZ2 (Residues: 1536-1850; Coverage: 7%)
Sequence domains: Coronavirus papain-like peptidase

Ligands and Environments

3 bound ligands:
Ligand 9OZ 1 x 9OZ
Ligand DMS 1 x DMS
Ligand ZN 2 x ZN
1 modified residue:
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6422
Unit cell:
a: 176.306Å b: 176.306Å c: 79.834Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Role of E3 ubiquitin ligases and deubiquitinating enzymes in SARS-CoV-2 infection.
Zhao et al. (2023)
2 other articles

1 mention without citation

Exploring Noncovalent Protease Inhibitors for the Treatment of Severe Acute Respiratory Syndrome and Severe Acute Respiratory Syndrome-Like Coronaviruses.
Freitas et al. (2022)