7s6n

X-ray diffraction
2.7Å resolution

N-acetylglucosamine-1-phosphotransferase (GNPT) alpha and beta subunits (GNPTAB) catalytic domain, from zebrafish

Released:
Source organism: Danio rerio
Primary publication:
Structures of the mannose-6-phosphate pathway enzyme, GlcNAc-1-phosphotransferase.
Proc Natl Acad Sci U S A 119 e2203518119 (2022)
PMID: 35939698

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-D-glucosamine + lysosomal-enzyme D-mannose = UMP + lysosomal-enzyme N-acetyl-D-glucosaminyl-phospho-D-mannose
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
N-acetylglucosamine-1-phosphotransferase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 495 amino acids
Theoretical weight: 58.36 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5RGJ8 (Residues: 893-1173; Coverage: 23%)
Gene names: gnpta, gnptab, si:ch211-234f20.3, zgc:122985
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, FUC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P21
Unit cell:
a: 97.182Å b: 86.605Å c: 106.985Å
α: 90° β: 112.169° γ: 90°
R-values:
R R work R free
0.186 0.184 0.231
Expression system: Spodoptera frugiperda