› 7ryc

Electron Microscopy
2.9Å resolution

Oxytocin receptor (OTR) bound to oxytocin in complex with a heterotrimeric Gq protein

Released:
DOI: 10.2210/pdb7ryc/pdb
PDB entry 7ryc coloured by chain, front view.
PDB entry 7ryc coloured by chain, side view.
PDB entry 7ryc coloured by chain, top view.
Source organisms:
Primary publication:
The oxytocin signaling complex reveals a molecular switch for cation dependence.
Meyerowitz JG, Robertson MJ, Barros-Álvarez X, Panova O, Nwokonko RM, Gao Y, Skiniotis G
Nat Struct Mol Biol 29 274-281 (2022)
PMID: 35241813
Related structures: EMD-24733

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208355 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Oxytocin Chain: L
Molecule details ›
Chain: L
Length: 10 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01178 (Residues: 20-28; Coverage: 9%)
Gene names: OT, OXT
Sequence domains: Neurohypophysial hormones, N-terminal Domain
Oxytocin receptor Chain: O
Molecule details ›
Chain: O
Length: 450 amino acids
Theoretical weight: 49.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30559 (Residues: 1-389; Coverage: 100%)
Gene name: OXTR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2; Guanine nucleotide-binding protein G(i) subunit alpha-2; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: D
Molecule details ›
Chain: D
Length: 326 amino acids
Theoretical weight: 36.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
  • Canonical: P04899 (Residues: 1-182, 310-310, 320-320, 348-349; Coverage: 20%)
  • Canonical: P63092 (Residues: 205-346, 348-357, 359-386, 389-394; Coverage: 45%)
Gene names: GNAI2, GNAI2B, GNAS, GNAS1, GNG2, GSP
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 260 amino acids
Theoretical weight: 27.72 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-24733
Expression systems:
  • Not provided
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

2 review citations

A journey from speech to dance through the field of oxytocin.
Theofanopoulou C. (2023)
1 more

4 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
3 more