› 7rws

X-ray diffraction
1.8Å resolution

Structure of SAVED domain of Cap5 from Lactococcus lactis in complex with cGAMP

Released:
DOI: 10.2210/pdb7rws/pdb
PDB entry 7rws coloured by chain, front view.
PDB entry 7rws coloured by chain, side view.
PDB entry 7rws coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris IBB477
Primary publication:
Molecular mechanisms of the CdnG-Cap5 antiphage defense system employing 3',2'-cGAMP as the second messenger.
Fatma S, Chakravarti A, Zeng X, Huang RH
Nat Commun 12 6381 (2021)
PMID: 34737303

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SMODS-associated and fused to various effectors domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 30.61 KDa
Source organism: Lactococcus lactis subsp. cremoris IBB477
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1E7G0A2 (Residues: 123-385; Coverage: 68%)
Gene name: AJ89_14580
Sequence domains: SMODS-associated and fused to various effectors sensor domain

Ligands and Environments

1 bound ligand:
Ligand 4UR 1 x 4UR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 68.696Å b: 126.713Å c: 83.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.191
Expression system: Escherichia coli

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Citations

6 review citations

Unveil the Secret of the Bacteria and Phage Arms Race.
Wang et al. (2023)
5 more