› 7rva

X-ray diffraction
1.89Å resolution

Updated Crystal Structure of Replication Initiator Protein REPE54.

Released:
DOI: 10.2210/pdb7rva/pdb
PDB entry 7rva coloured by chain, front view.
PDB entry 7rva coloured by chain, side view.
PDB entry 7rva coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Stabilizing DNA-Protein Co-Crystals via Intra-Crystal Chemical Ligation of the DNA
Ward AR, Dmytriw S, Vajapayajula A, Snow CD
Crystals 12 (2022)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-121936 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Replication initiation protein Chain: C
Molecule details ›
Chain: C
Length: 263 amino acids
Theoretical weight: 30.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03856 (Residues: 1-251; Coverage: 100%)
Gene names: E, ECOK12F045, rep, repE
Sequence domains:
UNK-UNK-UNK Chain: D
Molecule details ›
Chain: D
Length: 3 amino acids
Theoretical weight: 273 Da
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UNK-UNK-UNK-UNK Chain: E
Molecule details ›
Chain: E
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 6.69 KDa
DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.81 KDa

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 107.578Å b: 80.715Å c: 73.299Å
α: 90° β: 122.63° γ: 90°
R-values:
R R work R free
0.21 0.207 0.249
Expression system: Escherichia coli

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