Structure analysis

Structure of ribosomal complex bound with Rbg1/Tma46

Electron Microscopy
3.23Å resolution
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero 87-mer (preferred)
Entry contents: 80 distinct polypeptide molecules
7 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 87-mer
PDBe Complex ID: PDB-CPX-103550

Macromolecules

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Chain: LL
Length: 199 amino acids
Theoretical weight: 22.6 KDa
Source organism: Saccharomyces cerevisiae
InterPro:

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Chain: LS
Length: 172 amino acids
Theoretical weight: 20.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX23 (Residues: 1-172; Coverage: 100%)
Gene names: RPL18A, RPL18A2, RPL20A, YM9408.04C, YMR242C
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
InterPro:

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Chain: LU
Length: 121 amino acids
Theoretical weight: 13.71 KDa
Source organism: Saccharomyces cerevisiae
InterPro:

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Chain: LW
Length: 155 amino acids
Theoretical weight: 17.66 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PY83 (Residues: 1-155; Coverage: 100%)
Gene names: GI527_G0002390, RPL24A
Pfam: Ribosomal protein L24e
InterPro:

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Chain: Lb
Length: 59 amino acids
Theoretical weight: 6.69 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PWR1 (Residues: 1-59; Coverage: 100%)
Gene names: GI527_G0002126, RPL29
Pfam: Ribosomal L29e protein family
InterPro: Large ribosomal subunit protein eL29

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Chain: Le
Length: 130 amino acids
Theoretical weight: 14.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5Q1M9 (Residues: 1-130; Coverage: 100%)
Gene names: GI527_G0000109, RPL32
Pfam: Ribosomal protein L32
InterPro:

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Chain: Lg
Length: 121 amino acids
Theoretical weight: 13.67 KDa
Source organism: Saccharomyces cerevisiae
InterPro:

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Chain: Li
Length: 100 amino acids
Theoretical weight: 11.15 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05745 (Residues: 1-100; Coverage: 100%)
Gene names: RPL36A, RPL39A, YM9646.06, YMR194W
Pfam: Ribosomal protein L36e
InterPro:

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Chain: Lk
Length: 78 amino acids
Theoretical weight: 8.85 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PUL8 (Residues: 1-78; Coverage: 100%)
Gene names: GI527_G0004139, RPL38
Pfam: Ribosomal L38e protein family
InterPro:

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Chain: Ln
Length: 25 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX86 (Residues: 1-25; Coverage: 100%)
Gene names: D1290, RPL41A, RPL47A, YDL184C, YL41A
Pfam: Ribosomal protein L41
InterPro: Large ribosomal subunit protein eS32

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Chain: Lo
Length: 106 amino acids
Theoretical weight: 12.25 KDa
Source organism: Saccharomyces cerevisiae
InterPro:

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Chain: Lp
Length: 92 amino acids
Theoretical weight: 10.11 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX25 (Residues: 1-92; Coverage: 100%)
Gene names: RPL43A, YP9499.02, YPR043W
Pfam: Ribosomal L37ae protein family
InterPro:

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Chain: SB
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PRY4 (Residues: 1-255; Coverage: 100%)
Gene names: GI527_G0004262, RPS1, RPS1A
Pfam: Ribosomal S3Ae family
InterPro:

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Chain: SH
Length: 190 amino acids
Theoretical weight: 21.66 KDa
Source organism: Saccharomyces cerevisiae
InterPro:

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Chain: SV
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C0V8 (Residues: 1-87; Coverage: 100%)
Gene names: RPS21A, RPS25, RPS25A, RPS26A, YKR057W
Pfam: Ribosomal protein S21e
InterPro:

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Chain: SW
Length: 130 amino acids
Theoretical weight: 14.65 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PSE9 (Residues: 1-130; Coverage: 100%)
Gene names: GI527_G0003062, RPS22A
Pfam: Ribosomal protein S8
InterPro:

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Chain: SZ
Length: 108 amino acids
Theoretical weight: 12.07 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PZ18 (Residues: 1-108; Coverage: 100%)
Gene names: GI527_G0002448, RPS25A
Pfam: S25 ribosomal protein
InterPro:

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Chain: Sa
Length: 119 amino acids
Theoretical weight: 13.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5Q0E8 (Residues: 1-119; Coverage: 100%)
Gene names: GI527_G0001937, RPS26B
Pfam: Ribosomal protein S26e
InterPro:

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Chain: Sb
Length: 82 amino acids
Theoretical weight: 8.89 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35997 (Residues: 1-82; Coverage: 100%)
Gene names: RPS27A, YKL156W
Pfam: Ribosomal protein S27
InterPro:

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Chain: Sc
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5Q700 (Residues: 1-67; Coverage: 100%)
Gene names: GI527_G0005539, RPS28A
Pfam: Ribosomal protein S28e
InterPro:

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Chain: Se
Length: 63 amino acids
Theoretical weight: 7.14 KDa
Source organism: Saccharomyces cerevisiae
InterPro: Small ribosomal subunit protein eS30

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Chain: T
Length: 345 amino acids
Theoretical weight: 39.59 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5Q867 (Residues: 1-345; Coverage: 100%)
Gene names: GI527_G0005465, TMA46
Pfam: DRG Family Regulatory Proteins, Tma46
InterPro:

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Chain: L1
Length: 210 amino acids
Theoretical weight: 17.89 KDa
Source organism: Saccharomyces cerevisiae

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Name: 25S ribosomal RNA
Representative chains: C1
Length: 3396 nucleotides
Theoretical weight: 1097.49 KDa
Rfam: Eukaryotic large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: C4
Length: 121 nucleotides
Theoretical weight: 38.95 KDa
Rfam: 5S ribosomal RNA

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Name: 5.8S ribosomal RNA
Representative chains: C3
Length: 158 nucleotides
Theoretical weight: 50.68 KDa
Rfam: 5.8S ribosomal RNA

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Name: 18S Ribosomal RNA
Representative chains: C2
Length: 1800 nucleotides
Theoretical weight: 579.76 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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Name: A-tRNA
Representative chains: A
Length: 76 nucleotides
Theoretical weight: 24.36 KDa
Rfam: tRNA

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Name: P-tRNA
Representative chains: P
Length: 76 nucleotides
Theoretical weight: 24.45 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: m
Length: 16 nucleotides
Theoretical weight: 5.23 KDa

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