7rpb

X-ray diffraction
1.97Å resolution

X-ray crystal structure of OXA-24/40 V130D in complex with meropenem

Released:
Source organism: Acinetobacter baumannii
Entry authors: Powers RA, Mitchell JM, June CM

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transpeptidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 27.69 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8RLA6 (Residues: 31-275; Coverage: 97%)
Gene names: SI89_16690, bla-OXA-40, blaOXA-24, blaOXA-33, blaOXA-40, oxa-24, oxa40
Sequence domains: Penicillin binding protein transpeptidase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 102.391Å b: 102.391Å c: 85.647Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.229
Expression system: Escherichia coli BL21(DE3)