7rfc

X-ray diffraction
3.24Å resolution

Crystal structure of broadly neutralizing antibody mAb1382 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain

Released:
Source organisms:
Entry authors: Flyak AI, Bjorkman PJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
mAb1382 Heavy Chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 236 amino acids
Theoretical weight: 24.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
mAb1382 Light Chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 216 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
envelope glycoprotein E2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 262 amino acids
Theoretical weight: 28.95 KDa
Source organism: Hepacivirus C
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A2P0NE26 (Residues: 214-475; Coverage: 46%)
Sequence domains: Hepatitis C virus non-structural protein E2/NS1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 104.291Å b: 140.184Å c: 142.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.284
Expression system: Homo sapiens