› 7rc4

X-ray diffraction
1.66Å resolution

Aeronamide N-methyltransferase, AerE (D141A)

Released:
DOI: 10.2210/pdb7rc4/pdb
PDB entry 7rc4 coloured by chain, front view.
PDB entry 7rc4 coloured by chain, side view.
PDB entry 7rc4 coloured by chain, top view.
Source organism: Microvirgula aerodenitrificans DSM 15089
Primary publication:
Structure and mechanism for iterative amide N-methylation in the biosynthesis of channel-forming peptide cytotoxins.
Cogan DP, Bhushan A, Reyes R, Zhu L, Piel J, Nair SK
Proc Natl Acad Sci U S A 119 e2116578119 (2022)
PMID: 35316135

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104452 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase small domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 41.96 KDa
Source organism: Microvirgula aerodenitrificans DSM 15089
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2S0PEL5 (Residues: 1-384; Coverage: 100%)
Gene name: DAI18_18450
Sequence domains: Methyltransferase small domain

Ligands and Environments

5 bound ligands:
Ligand SAH 1 x SAH
Ligand NA 2 x NA
Ligand CA 1 x CA
Ligand PG4 1 x PG4
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3221
Unit cell:
a: 77.595Å b: 77.595Å c: 151.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.188
Expression system: Escherichia coli BL21(DE3)

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