7rbs

X-ray diffraction
2.98Å resolution

The crystal structure of Papain-Like Protease of SARS CoV-2, C111S mutant, in complex with human ISG15

Released:
Entry authors: Osipiuk J, Tesar C, Jedrzejczak R, Endres M, Wydorski P, Joachimiak L, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed:
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Papain-like protease nsp3 Chains: A, C, E, G, I
Molecule details ›
Chains: A, C, E, G, I
Length: 318 amino acids
Theoretical weight: 35.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTC1 (Residues: 1564-1878; Coverage: 7%)
Ubiquitin-like protein ISG15 Chains: B, D, F, H, J
Molecule details ›
Chains: B, D, F, H, J
Length: 159 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05161 (Residues: 2-157; Coverage: 95%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 153.673Å b: 220.87Å c: 232.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.236
Expression system: Escherichia coli BL21(DE3)