› 7r55

X-ray diffraction
1.84Å resolution

B-trefoil lectin from Salpingoeca rosetta in complex with Gb3

Released:
DOI: 10.2210/pdb7r55/pdb
PDB entry 7r55 coloured by chain, front view.
PDB entry 7r55 coloured by chain, side view.
PDB entry 7r55 coloured by chain, top view.
Source organism: Salpingoeca rosetta
Primary publication:
The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3.
Notova S, Bonnardel F, Rosato F, Siukstaite L, Schwaiger J, Lim JH, Bovin N, Varrot A, Ogawa Y, Römer W, Lisacek F, Imberty A
Commun Biol 5 954 (2022)
PMID: 36097056

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sarol-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.52 KDa
Source organism: Salpingoeca rosetta
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2UID9 (Residues: 1-329; Coverage: 100%)
Gene name: PTSG_08235

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL, GLA
Carbohydrate polymer
1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 57.341Å b: 58.997Å c: 210.306Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.24
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Taxonomic Distribution and Molecular Evolution of Mytilectins.
Gerdol et al. (2023)
3 other articles

1 mention without citation

The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3.
Notova et al. (2022)