7r4l

X-ray diffraction
2.6Å resolution

Crystal structure of human mitochondrial NAD kinase

Released:

Function and Biology Details

Reaction catalysed:
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase 2, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 44.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4G0N4 (Residues: 61-442; Coverage: 86%)
Gene names: C5orf33, MNADK, NADK2, NADKD1
Sequence domains: ATP-NAD kinase N-terminal domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 68.406Å b: 68.406Å c: 223.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli BL21(DE3)