7r3b

X-ray diffraction
2.82Å resolution

S-adenosylmethionine synthetase from Lactobacillus plantarum complexed with AMPPNP, methionine and SAM

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 401 amino acids
Theoretical weight: 43.53 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0G9F5E5 (Residues: 1-395; Coverage: 100%)
Gene names: AVR83_15510, AYO51_09395, C7M36_00090, C7M40_01013, E3O64_10015, E3U93_09955, FEE41_07350, IV39_GL000589, J8138_14495, LPJSA22_01228, Lp19_1604, Lp90_1155, Nizo2802_1764, SN35N_0475, metK
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 58.426Å b: 110.927Å c: 112.661Å
α: 93.82° β: 104.03° γ: 99.59°
R-values:
R R work R free
0.161 0.156 0.275
Expression system: Escherichia coli