7r2w

X-ray diffraction
1.6Å resolution

Mutant S-adenosylmethionine synthetase from E.coli complexed with AMPPNP and methionine

Released:
Source organism: Escherichia coli
Entry authors: Shahar A, Kleiner D, Bershtein S, Zarivach R

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: C3SV92 (Residues: 1-384; Coverage: 100%)
Gene names: A5U30_002527, A6592_01650, A8499_002478, A8502_001843, A9X72_04280, AAG43_002110, ACN68_17615, ACN81_19335, ACU57_02375, AF998_004760, AM464_06410, APX88_16490, AT335_001838, AT845_001710, AW118_05150, AW119_01670, AWP47_17420, B6R12_005057, B6R15_000194, B6R31_003803, B6R48_004372, B6R87_004190, BANRA_03830, BANRA_04027, BEA19_04635, BER14_05455, BF481_004834, BG944_003871, BGM66_000099, BHS81_17740, BJI68_00165, BK292_22170, BK383_12595, BKL28_001386, BLM69_002115, BMC79_000905, BMT49_18295, BMT50_15030, BMT91_06515, BN17_28551, BO068_000837, BOH76_01390, BON63_07270, BON64_00555, BON65_07810, BON66_18140, BON67_07025, BON68_25330, BON69_19675, BON70_26520, BON71_20775, BON72_22125, BON73_20740, BON74_12030, BON75_05765, BON76_19650, BON77_06630, BON80_08710, BON81_23645, BON82_19195, BON83_19430, BON84_07655, BON86_19275, BON87_03740, BON88_27610, BON89_07745, BON90_06505, BON91_21165, BON92_09240, BON93_14750, BON94_11580, BON95_23475, BON96_08705, BON97_01575, BON98_09380, BR158_001149, BRV02_002195, BTB68_004220, BTQ06_15250, BUO55_001088, BVL39_12010, BXT93_01450, BZL69_12445, BvCmsF30A_00766, BvCmsHHP056_00647, BvCmsKKP036_00795, BvCmsKKP061_01430, BvCmsKSNP073_01018, C0P57_003741, C1Q91_003943, C2121_000325, C2M16_21055, C2R31_001209, C2U48_19175, C3F40_21800, C5N07_11395, C5Y87_01860, C9114_13800, C9E67_04830, C9Z68_02900, CA593_12035, CCS08_04720, CCV12_004009, CDC27_15675, CDL36_12035, CDL37_10720, CF22_001577, CG831_001605, CHM61_002326, CIG67_09540, CO706_12725, CQ986_004760, CR538_04515, CR539_19835, CR628_000167, CTR35_003411, CV83915_03200, CWS33_02135, CX938_001268, CXJ73_003050, CY655_18815, D0X26_05680, D1912_20730, D1H34_002073, D3822_03935, D3C88_01260, D3G36_06460, D3Y67_09680, D4N09_02900, D4U49_10125, D9D43_09035, D9D77_01670, D9E34_07495, D9E49_12695, D9H94_01735, D9J03_20370, D9J61_17440, DAH17_01735, DAH18_09235, DAH19_03610, DAH20_07060, DAH21_16375, DAH22_01710, DAH23_02590, DAH24_02600, DAH25_06480, DAH26_02595, DAH27_13050, DAH28_13975, DAH29_08015, DAH30_03740, DAH31_07490, DAH32_08120, DAH33_07645, DAH35_09210, DAH36_04495, DAH37_13335, DAH38_14455, DAH40_03290, DAH41_08845, DAH42_03435, DAH43_04340, DAH45_02615, DAH46_04335, DAH47_01875, DAH48_00145, DAH49_05900, DAH50_00940, DD762_06715, DEN86_09100, DEN87_04935, DEN88_15300, DEN89_15405, DEN90_09945, DEN91_03005, DEN92_02600, DEN93_01850, DEN94_02810, DEN95_05350, DEN96_03305, DEN97_02960, DEN98_02965, DEN99_00320, DEO00_02305, DEO01_06440, DEO02_04085, DEO03_09990, DEO04_02005, DEO05_02000, DEO06_10445, DEO07_10850, DEO08_05190, DEO09_02005, DEO10_03905, DEO11_03920, DEO12_19525, DEO13_03760, DEO14_14520, DEO15_02615, DEO17_09215, DEO18_08460, DEO19_02740, DEO20_04925, DIV22_04770, DKP82_00480, DM870_04145, DN627_06545, DNQ45_23450, DNX30_22575, DRW19_08910, DS732_21800, DTL43_08520, DTL90_12520, DU321_13115, DXT69_09005, DXT70_01115, DXT71_16795, DXT73_11015, E0I42_11420, E2112_18590, E2113_22395, E2114_14280, E2115_10985, E2116_13525, E2117_06630, E2118_08195, E2119_17505, E2120_12560, E2121_10385, E2122_09245, E2123_07350, E2124_09540, E2125_11720, E2127_08745, E2128_15565, E2129_10820, E2130_12825, E2131_03825, E2132_07845, E2133_01745, E2134_10730, E2135_04050, E2136_04130, E2646_10705, E3N34_21965, E3O05_18105, E4K51_02055, E4T14_09220, E4T84_18290, E5H86_10815, E5M02_18320, E5P22_07460, E5P23_16365, E5P24_09460, E5P25_09305, E5P26_03105, E5P27_16455, E5P28_15040, E5P29_10030, E5P30_04620, E5P31_12535, E5P32_10655, E5P33_21455, E5P34_11405, E5P35_01415, E5P36_02995, E5P37_15650, E5P39_18665, E5P40_12060, E5P41_04075, E5P42_20280, E5P43_21945, E5P44_03710, E5P45_15450, E5P46_21780, E5P47_15090, E5P48_21170, E5P49_09740, E5P50_06555, E5P51_14675, E5P52_04900, E5S34_03760, E5S35_06215, E5S36_16645, E5S37_10335, E5S38_02955, E5S39_05685, E5S42_02360, E5S43_06565, E5S44_08325, E5S45_04380, E5S46_03005, E5S47_04080, E5S48_19810, E5S51_01000, E5S52_07415, E5S53_08910, E5S54_11470, E5S55_07870, E5S56_10450, E5S57_01330, E5S58_01560, E5S59_00710, E5S61_07030, E5S62_00730, E6C80_12510, E6D34_07775, EA239_10845, EA435_13710, EAI46_19880, EAN77_06295, EAX79_18805, EC95NR1_02239, ECs3818, ED648_12910, EHD79_07680, EI021_06040, EIA08_22380, EIZ93_04660, EKI52_19550, EL79_0780, EL80_0774, ELT16_22055, ELT17_05030, ELT20_11770, ELT21_06885, ELT22_16170, ELT23_09205, ELT24_14305, ELT25_23950, ELT26_01165, ELT27_12120, ELT28_18775, ELT29_11615, ELT30_23115, ELT31_10655, ELT32_11130, ELT33_10455, ELT34_21355, ELT35_11050, ELT36_22510, ELT38_09495, ELT39_24240, ELT40_23205, ELT41_09780, ELT44_23300, ELT45_23060, ELT46_09695, ELT48_18580, ELT49_03890, ELT50_07970, ELT51_11200, ELT52_07165, ELT54_23455, ELT55_20815, ELT56_08060, ELT58_03385, ELT59_22085, ELT60_23250, ELT61_23235, ELT63_26480, ELT72_21900, ELU07_18165, ELU82_21580, ELU83_15015, ELU85_04800, ELU88_08845, ELU89_22080, ELU90_06805, ELU91_22175, ELU94_16530, ELU95_07565, ELU96_24040, ELU97_24150, ELU98_12535, ELU99_15280, ELV00_09695, ELV01_14590, ELV02_23665, ELV03_14400, ELV04_23085, ELV05_13495, ELV07_18140, ELV08_03780, ELV10_14930, ELV11_25205, ELV12_21385, ELV13_23880, ELV15_23820, ELV16_20605, ELV20_21450, ELV21_16600, ELV22_07930, ELV23_09945, ELV26_14670, ELV28_10715, ELV29_20525, ELV40_03640, ELX48_15325, ELX61_10810, ELX66_14095, ELX68_24390, ELX69_09420, ELX76_01440, ELX79_17790, ELX85_03745, ELX96_00755, ELY02_06975, ELY31_13250, ELY32_13220, ELY36_14220, ELY39_02275, ELY41_00860, ELY48_11905, EN85_003901, ERS085406_01330, EWK56_04460, EXX13_15120, EYV17_02010, EYV18_21510, EYX47_12140, ExPECSC038_00146, F0L67_07320, F2N20_20245, F2N31_22105, F3N40_04310, F7F11_00240, F7N46_10945, F9413_05735, F9461_23100, F9B07_07340, F9S83_14665, F9V24_03825, F9X20_25295, FA849_12805, FA868_11850, FDM60_03845, FE584_00840, FE587_00840, FEJ01_00400, FEL34_10090, FFF58_11705, FGG80_12220, FHD44_04780, FHO90_01555, FHQ91_09185, FIJ20_02050, FJQ40_05790, FJQ51_10315, FJQ53_12830, FKO60_04490, FOI11_01765, FOI11_021415, FPI65_18145, FPJ29_01360, FPS11_11890, FQF29_10195, FV293_10510, FVB16_18150, FY127_18030, FZN31_04535, FZU14_09385, G3565_13785, G3813_002144, G3V95_09630, G3W53_04105, G4A38_13035, G4A47_13650, G5603_18860, G7630_000540, G9448_18900, GAI66_20650, GAI89_12615, GAJ12_01750, GAJ26_05450, GF147_11460, GF699_10820, GFY34_04960, GIB53_14095, GJ11_18975, GJO56_09225, GKF66_10900, GKF89_07990, GNW61_03995, GNZ05_07910, GOP25_09295, GP711_07520, GP944_11630, GP965_22940, GQM21_28990, GQN34_15645, GQW07_08095, GQW68_13955, GQW80_01070, GRC73_21435, GRO95_06370, GRW05_24700, GRW56_16030, GRW57_12880, GSM54_03540, GSY44_19485, GTP88_07990, GUC01_11385, GUI33_09300, H0O37_17935, H0O39_19520, H0O51_24410, H0O53_09000, H0O72_11130, H6Y26_001091, HCQ42_000085, HEP30_007450, HEP34_002642, HHH44_000266, HI055_000086, HIE29_001775, HIN64_001692, HJQ60_000361, HJS37_001146, HJU54_000350, HKA49_003344, HL563_05445, HL601_06650, HLV18_01565, HLX92_00625, HLZ50_19255, HMJ82_16495, HMU06_00775, HMV95_09340, HMW38_12015, HNC36_03025, HNC59_01765, HNC66_01755, HNC99_07950, HND12_16115, HV109_04035, HV209_16545, HV209_28475, HVV39_06610, HVW04_20335, HVW19_10750, HVW43_21595, HVX31_03730, HVY77_04635, HVZ30_03835, HVZ71_04415, HX136_04145, HmCms169_01458, I6H00_22985, I6H01_08635, I6H02_10200, IA00_000876, IFB95_002259, IH768_06200, IH772_17610, IT029_002527, J0541_004090, J4S20_000307, J5U05_000099, JE86ST02C_34150, JE86ST05C_34190, JFD_01481, JNP96_22280, NCTC10082_03261, NCTC10089_00907, NCTC10090_03962, NCTC10418_01419, NCTC10429_01518, NCTC10764_00069, NCTC10767_02118, NCTC10865_01132, NCTC11126_04797, NCTC11181_03113, NCTC12950_01072, NCTC13127_01214, NCTC13148_02906, NCTC13216_00978, NCTC4450_02848, NCTC7927_01050, NCTC8008_00345, NCTC8009_02196, NCTC8179_06427, NCTC8333_00995, NCTC8621_00893, NCTC8959_02673, NCTC8960_03582, NCTC9036_00921, NCTC9037_01064, NCTC9044_00787, NCTC9045_01025, NCTC9073_05156, NCTC9075_01376, NCTC9081_06061, NCTC9111_01306, NCTC9706_03130, NCTC9775_04655, NCTC9777_02402, NCTC9962_00203, ND22_003852, RG28_06710, SAMEA3472044_02863, SAMEA3472056_05183, SAMEA3472147_01437, SAMEA3751407_00790, SAMEA3752557_03413, SAMEA3753106_01289, TUM18780_07770, WP4S18E07_08670, WR15_12710, XU56_001681, metK, metK_1, metK_2
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 86.611Å b: 86.611Å c: 90.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli