› 7qru

Electron Microscopy
2.24Å resolution

Structure of Bacillus pseudofirmus Mrp antiporter complex, monomer

Released:
DOI: 10.2210/pdb7qru/pdb
PDB entry 7qru coloured by chain, front view.
PDB entry 7qru coloured by chain, side view.
PDB entry 7qru coloured by chain, top view.
Source organism: Alkalihalophilus pseudofirmus
Primary publication:
Ion transfer mechanisms in Mrp-type antiporters from high resolution cryoEM and molecular dynamics simulations.
Lee Y, Haapanen O, Altmeyer A, Kühlbrandt W, Sharma V, Zickermann V
Nat Commun 13 6091 (2022)
PMID: 36241630
Related structures: EMD-14124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-220230 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 493 amino acids
Theoretical weight: 54.61 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Q9PMS5 (Residues: 1-493; Coverage: 100%)
Gene name: BTR22_14495
Sequence domains: Proton-conducting membrane transporter
Na+/H+ antiporter subunit A Chain: A
Molecule details ›
Chain: A
Length: 805 amino acids
Theoretical weight: 89.3 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Q9PN15 (Residues: 1-805; Coverage: 100%)
Gene name: BTR22_14510
Sequence domains:
Na+/H+ antiporter MnhB subunit-related protein domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 144 amino acids
Theoretical weight: 15.75 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Q9PN06 (Residues: 1-144; Coverage: 100%)
Gene name: BTR22_14505
Sequence domains: Domain related to MnhB subunit of Na+/H+ antiporter
Na(+)/H(+) antiporter subunit C Chain: C
Molecule details ›
Chain: C
Length: 112 amino acids
Theoretical weight: 12.23 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RGZ3 (Residues: 1-112; Coverage: 100%)
Gene names: BpOF4_13200, mrpC
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Na+/H+ antiporter subunit E Chains: E, e
Molecule details ›
Chains: E, e
Length: 158 amino acids
Theoretical weight: 18.36 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1Q9PMT4 (Residues: 1-158; Coverage: 100%)
Gene name: BTR22_14490
Sequence domains: Na+/H+ ion antiporter subunit
Na(+)/H(+) antiporter subunit F Chain: F
Molecule details ›
Chain: F
Length: 91 amino acids
Theoretical weight: 10.02 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RGZ0 (Residues: 1-91; Coverage: 100%)
Gene names: BpOF4_13185, mrpF
Sequence domains: Multiple resistance and pH regulation protein F (MrpF / PhaF)
Na(+)/H(+) antiporter subunit G Chain: G
Molecule details ›
Chain: G
Length: 133 amino acids
Theoretical weight: 14.95 KDa
Source organism: Alkalihalophilus pseudofirmus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RGY9 (Residues: 1-119; Coverage: 100%)
Gene names: BpOF4_13180, mrpG
Sequence domains: Na+/H+ antiporter subunit

Ligands and Environments

1 bound ligand:
Ligand 3PE 3 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.24Å
Relevant EMDB volumes: EMD-14124
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From the 'black box' to 'domino effect' mechanism: what have we learned from the structures of respiratory complex I.
Sazanov LA. (2023)
1 other article