7qnb

Electron Microscopy
3.1Å resolution

Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210736 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Gamma-aminobutyric acid receptor subunit gamma-2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 487 amino acids
Theoretical weight: 55.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18507 (Residues: 51-475; Coverage: 98%)
Gene name: GABRG2
Sequence domains:
Gamma-aminobutyric acid receptor subunit beta-3 Chains: B, D, E
Molecule details ›
Chains: B, D, E
Length: 473 amino acids
Theoretical weight: 54.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 1-473; Coverage: 100%)
Gene name: GABRB3
Sequence domains:
Nanobody Nb25 Chain: N
Molecule details ›
Chain: N
Length: 121 amino acids
Theoretical weight: 13.34 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-14073
Expression systems:
  • Homo sapiens
  • Escherichia coli