› 7qiu

X-ray diffraction
1.88Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA (guanosine(2553)-2'-O)-methyltransferase RlmP Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.1 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P94538 (Residues: 1-248; Coverage: 100%)
Gene names: BSU28650, rlmP, ysgA
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 64.88Å b: 66.45Å c: 129.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.
Yelland et al. (2023)

1 mention without citation

The Bacillus subtilis open reading frame ysgA encodes the SPOUT methyltransferase RlmP forming 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA.
Roovers et al. (2022)