› 7qf3

X-ray diffraction
1.1Å resolution

Structure of the R57Q mutant of miniSOG expressed in E. coli in regular LB medium

Released:
DOI: 10.2210/pdb7qf3/pdb
PDB entry 7qf3 coloured by chain, front view.
PDB entry 7qf3 coloured by chain, side view.
PDB entry 7qf3 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Riboflavin-binding proteins for singlet oxygen production.
Lafaye C, Aumonier S, Torra J, Signor L, von Stetten D, Noirclerc-Savoye M, Shu X, Ruiz-González R, Gotthard G, Royant A, Nonell S
Photochem Photobiol Sci (2022)
PMID: 35041199

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
miniSOG (R57Q mutant) Chain: AAA
Molecule details ›
Chain: AAA
Length: 128 amino acids
Theoretical weight: 14.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

4 bound ligands:
Ligand FMN 1 x FMN
Ligand MG 1 x MG
Ligand CO 1 x CO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 39.951Å b: 39.951Å c: 133.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.17
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Riboflavin and Its Derivates as Potential Photosensitizers in the Photodynamic Treatment of Skin Cancers.
Insińska-Rak et al. (2023)
3 other articles

1 mention without citation

Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes.
Loviglio et al. (2017)