› 7q1e

X-ray diffraction
2.7Å resolution

CPAP:TUBULIN:IIH5 ALPHAREP COMPLEX

Released:
DOI: 10.2210/pdb7q1e/pdb
PDB entry 7q1e coloured by chain, front view.
PDB entry 7q1e coloured by chain, side view.
PDB entry 7q1e coloured by chain, top view.
Source organisms:
Primary publication:
Structural convergence for tubulin binding of CPAP and vinca domain microtubule inhibitors.
Campanacci V, Urvoas A, Ammar Khodja L, Aumont-Nicaise M, Noiray M, Lachkar S, Curmi PA, Minard P, Gigant B
OpenAccess logo Proc Natl Acad Sci U S A 119 e2120098119 (2022)
PMID: 35507869

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168644 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
iiH5 ALPHAREP Chains: C, D
Molecule details ›
Chains: C, D
Length: 170 amino acids
Theoretical weight: 18.87 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Centromere protein J Chain: P
Molecule details ›
Chain: P
Length: 79 amino acids
Theoretical weight: 9.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HC77 (Residues: 319-397; Coverage: 6%)
Gene names: CENPJ, CPAP, LAP, LIP1

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 53.59Å b: 68.05Å c: 420.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Computational Approaches to the Rational Design of Tubulin-Targeting Agents.
Pérez-Peña et al. (2023)