› 7plu

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the actomyosin-V complex in the rigor state (central 3er/2er)

Released:
DOI: 10.2210/pdb7plu/pdb
PDB entry 7plu coloured by chain, front view.
PDB entry 7plu coloured by chain, side view.
PDB entry 7plu coloured by chain, top view.
Source organisms:
Primary publication:
High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Pospich S, Sweeney HL, Houdusse A, Raunser S
Elife 10 (2021)
PMID: 34812732
Related structures: EMD-13502

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-147011 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Unconventional myosin-Va Chains: A, D
Molecule details ›
Chains: A, D
Length: 792 amino acids
Theoretical weight: 91.36 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02440 (Residues: 1-792; Coverage: 43%)
Gene name: MYO5A
Sequence domains:
Actin, alpha skeletal muscle Chains: C, F, G
Molecule details ›
Chains: C, F, G
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Phalloidin Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: Amanita phalloides
Expression system: Not provided
Myosin light chain 6B Chains: B, E
Molecule details ›
Chains: B, E
Length: 151 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14649 (Residues: 58-208; Coverage: 73%)
Gene names: MLC1SA, MYL6B

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand MG 3 x MG
3 modified residues:
Ligand HIC 3 x HIC
Ligand DTH 3 x DTH
Ligand HYP 3 x HYP

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-13502
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

3 review citations

Actin polymerization and depolymerization in developing vertebrates.
Bai et al. (2023)
2 more

1 mention without citation

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Pospich et al. (2021)