7pji

X-ray diffraction
1.65Å resolution

Crystal structure of Pseudomonas aeruginosa guaB (IMP dehydrogenase) bound to ATP and GDP at 1.65A resolution

Released:
Source organism: Pseudomonas aeruginosa
Entry authors: Fernandez-Justel D, Buey RM

Function and Biology Details

Reaction catalysed:
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 492 amino acids
Theoretical weight: 52.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A069Q6I8 (Residues: 1-489; Coverage: 100%)
Gene names: CAZ10_17565, CGU42_10690, CSB93_2499, DT376_00630, DY940_09800, E4V10_03770, E5D53_20830, E5Z62_00355, E5Z63_24315, ECC04_008545, GNQ48_05465, GUL26_01290, IPC111_14770, IPC112_14945, IPC113_02990, IPC114_12560, IPC115_02990, IPC116_03070, IPC117_05545, IPC118_03070, IPC119_05545, IPC120_02725, IPC121_02660, IPC122_03070, IPC123_07875, IPC124_22145, IPC125_19045, IPC126_15405, IPC127_01295, IPC128_02275, IPC1295_13460, IPC129_05870, IPC1306_13280, IPC1307_19885, IPC1308_10455, IPC1309_14225, IPC130_02275, IPC1310_08415, IPC1311_01305, IPC1312_19385, IPC1314_11585, IPC1315_03000, IPC1316_17390, IPC1317_14940, IPC1318_04135, IPC1319_18035, IPC131_12570, IPC1320_25975, IPC1321_04390, IPC1322_03035, IPC1323_03035, IPC1324_03035, IPC1325_18735, IPC1326_22355, IPC1327_22335, IPC1328_03025, IPC1329_18525, IPC132_13580, IPC1330_03030, IPC1331_02360, IPC1332_03365, IPC1333_17035, IPC1334_03080, IPC1335_00295, IPC1336_04575, IPC1337_04895, IPC1338_02810, IPC1339_03115, IPC133_05565, IPC1340_07665, IPC1341_03195, IPC1342_06305, IPC1343_16475, IPC1345_12520, IPC1346_08390, IPC1347_11235, IPC1348_08445, IPC1349_19215, IPC134_09820, IPC135_05565, IPC137_11645, IPC139_10510, IPC140_13425, IPC141_17885, IPC142_18010, IPC143_15580, IPC144_15335, IPC145_11970, IPC146_20430, IPC1474_10935, IPC1476_13960, IPC1477_19720, IPC1478_12970, IPC1479_29740, IPC147_06725, IPC1480_29875, IPC1481_30840, IPC1482_25400, IPC1485_03530, IPC1486_05215, IPC1487_04995, IPC1488_04040, IPC1489_01825, IPC148_16065, IPC1490_06680, IPC1491_16165, IPC1492_09380, IPC1494_18130, IPC1495_05990, IPC1496_03035, IPC1498_07380, IPC1499_08210, IPC149_10475, IPC1500_16475, IPC1501_18200, IPC1502_01660, IPC1503_23345, IPC1504_19315, IPC1505_09885, IPC1506_02040, IPC1507_12510, IPC1508_03345, IPC1509_02385, IPC150_02390, IPC1510_18565, IPC1511_05760, IPC1512_09250, IPC1513_03030, IPC1514_07300, IPC1515_05565, IPC1516_05490, IPC1517_18245, IPC1518_09050, IPC1519_03170, IPC151_02390, IPC1520_16755, IPC1521_03380, IPC1522_24540, IPC1523_06645, IPC152_04635, IPC153_12840, IPC154_13645, IPC155_09925, IPC156_15780, IPC157_03320, IPC1583_03000, IPC1584_21920, IPC1587_09160, IPC1588_06605, IPC1589_17850, IPC158_18710, IPC1591_05440, IPC1592_05570, IPC1593_02050, IPC1594_07720, IPC1595_07665, IPC1596_01305, IPC1597_01305, IPC1598_12600, IPC1599_07210, IPC159_13580, IPC1600_09395, IPC1601_02010, IPC1602_03030, IPC1603_23775, IPC1604_12610, IPC1605_18225, IPC1606_15250, IPC161_18955, IPC162_14095, IPC163_08035, IPC164_12910, IPC165_08040, IPC166_09735, IPC167_08040, IPC168_04545, IPC169_08035, IPC170_12775, IPC171_07305, IPC172_07655, IPC173_09385, IPC174_13950, IPC175_02010, IPC176_09145, IPC177_06700, IPC178_17840, IPC179_20080, IPC180_22290, IPC181_24990, IPC182_10975, IPC183_10225, IPC184_13150, IPC26_01615, IPC27_08340, IPC29_11705, IPC30_07765, IPC31_06430, IPC32_05485, IPC33_10715, IPC34_19055, IPC35_19225, IPC36_21115, IPC37_05315, IPC38_01335, IPC40_01405, IPC41_04025, IPC42_07995, IPC43_10780, IPC44_07345, IPC45_15295, IPC46_16205, IPC47_17255, IPC48_04005, IPC49_17630, IPC50_13805, IPC51_10525, IPC54_12725, IPC55_10090, IPC56_06190, IPC574_21060, IPC575_17240, IPC576_16015, IPC577_17415, IPC578_19205, IPC579_16850, IPC57_09045, IPC580_24275, IPC582_15390, IPC584_17725, IPC586_20760, IPC589_18290, IPC58_04520, IPC596_05500, IPC597_12415, IPC598_03155, IPC599_05500, IPC59_05725, IPC600_05505, IPC601_05495, IPC602_05505, IPC603_11615, IPC604_24405, IPC605_25585, IPC606_27710, IPC607_02300, IPC608_11685, IPC609_18565, IPC60_09570, IPC610_12795, IPC611_25190, IPC612_29330, IPC613_28005, IPC614_27145, IPC615_20205, IPC616_16145, IPC618_08655, IPC61_10900, IPC620_11170, IPC621_12315, IPC622_05500, IPC623_03030, IPC624_23110, IPC625_05610, IPC627_05505, IPC629_05620, IPC630_12570, IPC632_09420, IPC633_17690, IPC634_16155, IPC64_11805, IPC65_10225, IPC66_15430, IPC67_25985, IPC68_14230, IPC70_02400, IPC71_02010, IPC72_05555, IPC737_18950, IPC73_25045, IPC74_25655, IPC75_19215, IPC76_19215, IPC77_15585, IPC78_17180, NCTC12924_06429, NCTC13621_00582, PA52Ts2_5150, PAMH19_1311, guaB
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I4
Unit cell:
a: 120.947Å b: 120.947Å c: 145.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.195
Expression system: Escherichia coli BL21(DE3)