7pec

Electron Microscopy
4.24Å resolution

cryo-EM structure of DEPTOR bound to human mTOR complex 1, DEPt-bound subset local refinement

Released:
Source organism: Homo sapiens
Related structures: EMD-13352

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154625 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein kinase mTOR Chain: A
Molecule details ›
Chain: A
Length: 2549 amino acids
Theoretical weight: 287.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chain: C
Molecule details ›
Chain: C
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Regulatory-associated protein of mTOR Chain: E
Molecule details ›
Chain: E
Length: 1396 amino acids
Theoretical weight: 155.96 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:
DEP domain-containing mTOR-interacting protein Chain: I
Molecule details ›
Chain: I
Length: 409 amino acids
Theoretical weight: 46.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TB45 (Residues: 1-409; Coverage: 100%)
Gene names: DEPDC6, DEPTOR
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.24Å
Relevant EMDB volumes: EMD-13352
Expression system: Spodoptera frugiperda