X-ray diffraction
1.35Å resolution

BurG E232Q mutant (holo) in complex with enol-oxalacetate (15): Biosynthesis of cyclopropanol rings in bacterial toxins


Function and Biology Details

Reaction catalysed:
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Ketol-acid reductoisomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 38.72 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q2T3G7 (Residues: 2-353; Coverage: 100%)
Gene names: BTH_II2094, ilvC-2
Sequence domains:

Ligands and Environments

Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 58.29Å b: 76.03Å c: 78.23Å
α: 90° β: 106.14° γ: 90°
R R work R free
0.157 0.156 0.18
Expression system: Escherichia coli BL21(DE3)