› 7p9o

X-ray diffraction
2.1Å resolution

Structure of E.coli RlmJ in complex with a SAM analogue (CA)

Released:
DOI: 10.2210/pdb7p9o/pdb
PDB entry 7p9o coloured by chain, front view.
PDB entry 7p9o coloured by chain, side view.
PDB entry 7p9o coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Synthesis of RNA-cofactor conjugates and structural exploration of RNA recognition by an m6A RNA methyltransferase.
Meynier V, Iannazzo L, Catala M, Oerum S, Braud E, Atdjian C, Barraud P, Fonvielle M, Tisné C, Ethève-Quelquejeu M
Nucleic Acids Res 50 5793-5806 (2022)
PMID: 35580049

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(2030) in 23S rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase J Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.99 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37634 (Residues: 1-280; Coverage: 100%)
Gene names: JW3466, b3499, rlmJ, yhiR
Sequence domains: Ribosomal RNA large subunit methyltransferase D, RlmJ

Ligands and Environments


Cofactor: Ligand 0Y0 1 x 0Y0
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 79.99Å b: 38.89Å c: 90.65Å
α: 90° β: 105.62° γ: 90°
R-values:
R R work R free
0.243 0.24 0.306
Expression system: Escherichia coli BL21(DE3)

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