7p90

X-ray diffraction
1.87Å resolution

Crystal structure of Paradendryphiella salina PL7A alginate lyase in complex with tetra-mannuronic acid products

Released:
Source organism: Paradendryphiella salina
Entry authors: Fredslund F, Welner DH, Wilkens C

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-105113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 25.47 KDa
Source organism: Paradendryphiella salina
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A485PVH1 (Residues: 23-243; Coverage: 100%)
Gene name: PsAlg7A
Sequence domains: Alginate lyase

Ligands and Environments

Carbohydrate polymer : NEW Components: BEM, MAW
Carbohydrate polymer : NEW Components: BEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P212121
Unit cell:
a: 35.16Å b: 80.92Å c: 81.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.21
Expression system: Komagataella pastoris