› 7p5w

Electron Microscopy
3.5Å resolution

Structure of homomeric LRRC8A Volume-Regulated Anion Channel in complex with synthetic nanobody Sb2

Released:
DOI: 10.2210/pdb7p5w/pdb
PDB entry 7p5w coloured by chain, front view.
PDB entry 7p5w coloured by chain, side view.
PDB entry 7p5w coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains.
Deneka D, Rutz S, Hutter CAJ, Seeger MA, Sawicka M, Dutzler R
Nat Commun 12 5435 (2021)
PMID: 34521847
Related structures: EMD-13203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Volume-regulated anion channel subunit LRRC8A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 810 amino acids
Theoretical weight: 94.24 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q80WG5 (Residues: 1-1, 15-808; Coverage: 98%)
Gene names: Lrrc8, Lrrc8a
Sequence domains:
synthetic nanobody Sb2 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 150 amino acids
Theoretical weight: 16.75 KDa
Source organism: synthetic construct
Expression system: Escherichia coli MC1061

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-13203
Expression systems:
  • Homo sapiens
  • Escherichia coli MC1061

Quick links

Citations

2 review citations

Properties, Structures, and Physiological Roles of Three Types of Anion Channels Molecularly Identified in the 2010's.
Okada et al. (2021)
1 more

2 mentions without citation

Recent Advances in the Structural Biology of the Volume-Regulated Anion Channel LRRC8.
Kasuya et al. (2022)
1 more