› 7p0h

X-ray diffraction
2.5Å resolution

Crystal structure of Helicobacter pylori ComF fused to an artificial alphaREP crystallization helper(named B2)

Released:
DOI: 10.2210/pdb7p0h/pdb
PDB 7p0h coloured by chain and viewed from the front.
PDB 7p0h coloured by chain and viewed from the side.
PDB 7p0h coloured by chain and viewed from the top.
Source organism: synthetic construct
Primary publication:
ComFC mediates transport and handling of single-stranded DNA during natural transformation.
Damke PP, Celma L, Kondekar SM, Di Guilmi AM, Marsin S, Depagne J, Veaute X, Legrand P, Walbott H, Vercruyssen J, Guerois R, Quevillon-Cheruel S, Radicella JP
Nat Commun 13 1961-1961 (2022)
PMID: 35414142

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ComF family protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 427 amino acids
Theoretical weight: 47.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O26008 (Residues: 1-191; Coverage: 100%)
Gene name: HP_1473

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand PRP 4 x PRP
Ligand MG 8 x MG
Ligand GOL 14 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 57.88Å b: 87.98Å c: 122.79Å
α: 80.23° β: 76.44° γ: 76.39°
R-values:
R R work R free
0.222 0.221 0.245
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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