› 7oua

X-ray diffraction
2.09Å resolution

Crystal structure of dimeric chlorite dismutase variant R127K (CCld R127K) from Cyanothece sp. PCC7425

Released:
DOI: 10.2210/pdb7oua/pdb
PDB entry 7oua coloured by chain, front view.
PDB entry 7oua coloured by chain, side view.
PDB entry 7oua coloured by chain, top view.
Source organism: Cyanothece sp. PCC 7425
Primary publication:
Impact of the dynamics of the catalytic arginine on nitrite and chlorite binding by dimeric chlorite dismutase.
Serra I, Schmidt D, Pfanzagl V, Mlynek G, Hofbauer S, Djinović-Carugo K, Furtmüller PG, García-Rubio I, Van Doorslaer S, Obinger C
J Inorg Biochem 227 111689 (2022)
PMID: 34922158

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chlorite dismutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.8 KDa
Source organism: Cyanothece sp. PCC 7425
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8HNS6 (Residues: 2-182; Coverage: 100%)
Gene name: Cyan7425_1434
Sequence domains: Chlorite dismutase

Ligands and Environments

6 bound ligands:
Ligand HEM 2 x HEM
Ligand GOL 1 x GOL
Ligand MES 2 x MES
Ligand PGE 3 x PGE
Ligand SO4 1 x SO4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 48.712Å b: 49.607Å c: 54.579Å
α: 102.484° β: 104.838° γ: 105.002°
R-values:
R R work R free
0.225 0.222 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Roles of High-valent Hemes and pH Dependence in Halite Decomposition Catalyzed by Chlorite Dismutase from Dechloromonas aromatica.
Geeraerts et al. (2022)
1 more