› 7opp

X-ray diffraction
2.32Å resolution

Crystal structure of the Rab27a fusion with Slp2a-RBDa1 effector for SF4 pocket drug targeting

Released:
DOI: 10.2210/pdb7opp/pdb
PDB entry 7opp coloured by chain, front view.
PDB entry 7opp coloured by chain, side view.
PDB entry 7opp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of the first structurally validated covalent ligands of the small GTPase RAB27A.
Jamshidiha M, Lanyon-Hogg T, Sutherell CL, Craven GB, Tersa M, De Vita E, Brustur D, Pérez-Dorado I, Hassan S, Petracca R, Morgan RM, Sanz-Hernández M, Norman JC, Armstrong A, Mann DJ, Cota E, Tate EW
RSC Med Chem 13 150-155 (2022)
PMID: 35308027

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-27A; Synaptotagmin-like protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 230 amino acids
Theoretical weight: 25.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HCH5 (Residues: 5-32; Coverage: 3%)
  • Canonical: P51159 (Residues: 1-192; Coverage: 87%)
Gene names: KIAA1597, RAB27, RAB27A, SGA72M, SLP2, SLP2A, SYTL2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GNP 2 x GNP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 117.71Å b: 117.71Å c: 115.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.201
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Reactivity of Covalent Fragments and Their Role in Fragment Based Drug Discovery.
McAulay et al. (2022)
1 more

1 mention without citation

Identification of the first structurally validated covalent ligands of the small GTPase RAB27A.
Jamshidiha et al. (2022)