7om6

X-ray diffraction
2.18Å resolution

Thosea asigna virus RdRP domain in complex with RNA

Released:
Primary publication:
Snapshots of a Non-Canonical RdRP in Action.
Viruses 13 (2021)
PMID: 34203380

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA-dependent RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 684 amino acids
Theoretical weight: 77.11 KDa
Source organism: Thosea asigna virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6A562 (Residues: 11-671; Coverage: 53%)
Gene name: RdRp
RNA (5'-R(P*AP*AP*AP*UP*UP*U)-3') Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 8 nucleotides
Theoretical weight: 2.5 KDa
Source organism: synthetic construct
Expression system: synthetic construct

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 65.636Å b: 204.028Å c: 70.076Å
α: 90° β: 97.19° γ: 90°
R-values:
R R work R free
0.229 0.229 0.241
Expression systems:
  • Escherichia coli BL21
  • synthetic construct