› 7oi1

X-ray diffraction
1.9Å resolution

Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase

Released:
DOI: 10.2210/pdb7oi1/pdb
PDB entry 7oi1 coloured by chain, front view.
PDB entry 7oi1 coloured by chain, side view.
PDB entry 7oi1 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Discovery of a Ni2+-dependent guanidine hydrolase in bacteria.
Funck D, Sinn M, Fleming JR, Stanoppi M, Dietrich J, López-Igual R, Mayans O, Hartig JS
Nature 603 515-521 (2022)
PMID: 35264792

Function and Biology Details

Reaction catalysed: 
Agmatine + H(2)O = putrescine + urea
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable agmatinase 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 392 amino acids
Theoretical weight: 43.14 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P73270 (Residues: 1-390; Coverage: 100%)
Gene names: sll1077, speB2
Sequence domains: Arginase family

Ligands and Environments

5 bound ligands:
Ligand CL 2 x CL
Ligand NI 6 x NI
Ligand CAC 3 x CAC
Ligand EDO 5 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 102.36Å b: 140.21Å c: 86.85Å
α: 90° β: 119.75° γ: 90°
R-values:
R R work R free
0.174 0.173 0.208
Expression system: Escherichia coli

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Citations

1 review citation

Still rocking in the structural era: A molecular overview of the small multidrug resistance (SMR) transporter family.
Burata et al. (2022)
7 other articles