7oh7

Electron Microscopy
3.8Å resolution

Cryo-EM structure of Drs2p-Cdc50p in the E1-AMPPCP state with PI4P bound

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Probable phospholipid-transporting ATPase DRS2 Chain: A
Molecule details ›
Chain: A
Length: 1465 amino acids
Theoretical weight: 164.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39524 (Residues: 1-1246, 1247-1355; Coverage: 100%)
Gene names: DRS2, FUN38, YAL026C
Sequence domains:
Cell division control protein 50 Chain: C
Molecule details ›
Chain: C
Length: 413 amino acids
Theoretical weight: 47.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25656 (Residues: 1-391; Coverage: 100%)
Gene names: CDC50, YCR094W, YCR94W
Sequence domains: LEM3 (ligand-effect modulator 3) family / CDC50 family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-12896
Expression system: Saccharomyces cerevisiae