› 7ogr

Electron Microscopy
3Å resolution

Structure of the apo-state of the bacteriophage PhiKZ non-virion RNA polymerase

Released:
DOI: 10.2210/pdb7ogr/pdb
PDB entry 7ogr coloured by chain, front view.
PDB entry 7ogr coloured by chain, side view.
PDB entry 7ogr coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the bacteriophage PhiKZ non-virion RNA polymerase.
deYMartín Garrido N, Orekhova M, Lai Wan Loong YTE, Litvinova A, Ramlaul K, Artamonova T, Melnikov AS, Serdobintsev P, Aylett CHS, Yakunina M
Nucleic Acids Res 49 7732-7739 (2021)
PMID: 34181731
Related structures: EMD-12886

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-125234 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
UNK helices Chain: X
Molecule details ›
Chain: X
Length: 32 amino acids
Theoretical weight: 2.74 KDa
Source organism: Pseudomonas phage phiKZ
Expression system: Escherichia coli
DNA-directed RNA polymerase; PHIKZ056.1 Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 57.98 KDa
Source organism: Pseudomonas phage phiKZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SDA7 (Residues: 1-419; Coverage: 100%)
  • Canonical: L7T138 (Residues: 1-61; Coverage: 100%)
PHIKZ068 Chain: B
Molecule details ›
Chain: B
Length: 521 amino acids
Theoretical weight: 59.42 KDa
Source organism: Pseudomonas phage phiKZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SD94 (Residues: 1-521; Coverage: 100%)
DNA-directed RNA polymerase; PHIKZ071 Chain: C
Molecule details ›
Chain: C
Length: 700 amino acids
Theoretical weight: 78.78 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SD91 (Residues: 1-393; Coverage: 100%)
  • Canonical: I7DB36 (Residues: 1-300; Coverage: 100%)
PHIKZ074 Chain: D
Molecule details ›
Chain: D
Length: 677 amino acids
Theoretical weight: 77.51 KDa
Source organism: Pseudomonas phage phiKZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SD88 (Residues: 1-677; Coverage: 100%)
PHIKZ123 Chain: E
Molecule details ›
Chain: E
Length: 543 amino acids
Theoretical weight: 62.96 KDa
Source organism: Pseudomonas phage phiKZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SD39 (Residues: 1-543; Coverage: 100%)

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-12886
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.
Prichard et al. (2023)
4 more

2 mentions without citation

Structure of the bacteriophage PhiKZ non-virion RNA polymerase.
deYMartín Garrido et al. (2021)
1 more