› 7odf

Electron Microscopy
2.7Å resolution

Structure of the mini-RNA-guided endonuclease CRISPR-Cas_phi3

Released:
DOI: 10.2210/pdb7odf/pdb
PDB entry 7odf coloured by chain, front view.
PDB entry 7odf coloured by chain, side view.
PDB entry 7odf coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3.
Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G
Nat Commun 12 4476 (2021)
PMID: 34294706
Related structures: EMD-12827

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-121723 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cas_phi3 Chain: A
Molecule details ›
Chain: A
Length: 766 amino acids
Theoretical weight: 87.41 KDa
Source organism: Phage #D
Expression system: Escherichia coli
DNA (5'-D(P*GP*TP*AP*TP*CP*CP*CP*AP*TP*TP*AP*CP*CP*AP*GP*CP*TP*GP*AP*AP*TP*TP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.29 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*AP*TP*TP*CP*AP*G)-3') Chain: G
Molecule details ›
Chain: G
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 2 nucleotides
Theoretical weight: 613 Da
Source organism: synthetic construct
Expression system: Not provided
RNA (43-MER) Chain: F
Molecule details ›
Chain: F
Length: 43 nucleotides
Theoretical weight: 13.85 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-12827
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises.
Rasheed et al. (2021)
7 more

2 mentions without citation

Miniature CRISPR-Cas12 endonucleases - Programmed DNA targeting in a smaller package.
Nguyen et al. (2022)
1 more