› 7obs

X-ray diffraction
1.8Å resolution

Crystal structure of 14-3-3 sigma in complex with RIPK2 phosphopeptide

Released:
DOI: 10.2210/pdb7obs/pdb
PDB entry 7obs coloured by chain, front view.
PDB entry 7obs coloured by chain, side view.
PDB entry 7obs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
IFNα primes cancer cells for Fusicoccin-induced cell death via 14-3-3 PPI stabilization.
Andlovic B, Heilmann G, Ninck S, Andrei SA, Centorrino F, Higuchi Y, Kato N, Brunsveld L, Arkin M, Menninger S, Choidas A, Wolf A, Klebl B, Kaschani F, Kaiser M, Eickhoff J, Ottmann C
Cell Chem Biol (2023)
PMID: 37130519

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128910 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Receptor-interacting serine/threonine-protein kinase 2 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43353 (Residues: 530-540; Coverage: 2%)
Gene names: CARDIAK, RICK, RIP2, RIPK2, UNQ277/PRO314/PRO34092

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MG 3 x MG
2 modified residues:
Ligand CSO 1 x CSO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: C2221
Unit cell:
a: 82.612Å b: 111.816Å c: 62.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.189
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Structure-Based Optimization of Covalent, Small-Molecule Stabilizers of the 14-3-3σ/ERα Protein-Protein Interaction from Nonselective Fragments.
Konstantinidou et al. (2023)