7o8t

X-ray diffraction
2.5Å resolution

NmHR light state structure at 37.5 ms (35 - 40 ms) after photoexcitation determined by serial millisecond crystallography

Released:
Source organism: Nonlabens marinus S1-08
Entry authors: Mous S, Gotthard G, Ehrenberg D, Sen S, James D, Johnson P, Weinert T, Nass K, Furrer A, Kekilli D, Ma P, Bruenle S, Casadei C, Martiel I, Dworkowski F, Gashi D, Skopintsev P, Wranik M, Knopp G, Panepucci E, Panneels V, Cirelli C, Ozerov D, Schertler G, Wang M, Milne C, Standfuss J, Schapiro I, Heberle J, Nogly P

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.91 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 104.48Å b: 51.18Å c: 78.33Å
α: 90° β: 131.78° γ: 90°
R-values:
R R work R free
0.283 0.281 0.325
Expression system: Escherichia coli BL21(DE3)