› 7o8m

X-ray diffraction
2.2Å resolution

NmHR light state structure at 2.5 ms (0 - 5 ms) after photoexcitation determined by serial millisecond crystallography

Released:
DOI: 10.2210/pdb7o8m/pdb
PDB 7o8m coloured by chain and viewed from the front.
PDB 7o8m coloured by chain and viewed from the side.
PDB 7o8m coloured by chain and viewed from the top.
Source organism: Nonlabens marinus S1-08
Primary publication:
Dynamics and mechanism of a light-driven chloride pump.
Mous S, Gotthard G, Ehrenberg D, Sen S, Weinert T, Johnson PJM, James D, Nass K, Furrer A, Kekilli D, Ma P, Brünle S, Casadei CM, Martiel I, Dworkowski F, Gashi D, Skopintsev P, Wranik M, Knopp G, Panepucci E, Panneels V, Cirelli C, Ozerov D, Schertler GFX, Wang M, Milne C, Standfuss J, Schapiro I, Heberle J, Nogly P
Science eabj6663 (2022)
PMID: 35113649

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.91 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand OLC 3 x OLC
Ligand OLA 6 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 104.48Å b: 51.18Å c: 78.33Å
α: 90° β: 131.78° γ: 90°
R-values:
R R work R free
0.238 0.236 0.28
Expression system: Escherichia coli BL21(DE3)

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