7o84

X-ray diffraction
2.18Å resolution

Structure of the PL6 family alginate lyase Pedsa0632 from Pseudopedobacter saltans in complex with substrate

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alginate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 47.45 KDa
Source organism: Pseudopedobacter saltans DSM 12145
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F0S7Y7 (Residues: 23-448; Coverage: 100%)
Gene name: Pedsa_0632
Sequence domains: Chondroitinase B

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU, MAW
Carbohydrate polymer : NEW Components: LGU, MAW
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 81.182Å b: 47.583Å c: 116.2Å
α: 90° β: 98.25° γ: 90°
R-values:
R R work R free
0.231 0.228 0.286
Expression system: Escherichia coli BL21(DE3)